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Studies on the performance of different coiled column configurations for compact type‐I countercurrent chromatography
Author(s) -
Yang Yi,
Gu Dongyu,
Aisa Haji Akber,
Ito Yoichiro
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100019
Subject(s) - countercurrent chromatography , chemistry , chromatography , solvent , hydrochloric acid , countercurrent distribution , acetic acid , countercurrent exchange , column chromatography , analytical chemistry (journal) , hexane , organic chemistry , physics , thermodynamics
Three types of novel coiled column configurations, i.e. a triangular coiled column and elliptical coiled columns I and II, were designed for type‐I countercurrent chromatography and their performances were evaluated with two solvent systems each with suitable test samples. Three dinitrophenyl (DNP) amino acids (DNP‐ DL ‐glu, DNP‐β‐ala and DNP‐ L ‐ala) were separated with a moderately hydrophobic two‐phase solvent system composed of hexane–ethyl acetate–metanol–0.1 M hydrochloric acid (1:1:1:1, v/v), while two dipeptides (tryptophyl‐tyrosine and valyl‐tyrosine) were separated with a polar solvent system composed of 1‐butanol–acetic acid–water (4.75:0.25:5, v/v). The overall results indicated that the performance of compact type‐I countercurrent chromatography was improved by elliptical coiled column I which was mounted with its maximum coil diameter perpendicular to the surface of the column holder. Hydrodynamic effects involved in these separations were discussed.