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Synthesis and study of a molecularly imprinted polymer for specific solid‐phase extraction of vinflunine and its metabolite from biological fluids
Author(s) -
Lopez Claire,
Claude Bérengère,
Morin Philippe,
Pelissou Martine,
Pena Richard,
Max JeanPaul,
Ribet JeanPaul
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100015
Subject(s) - molecularly imprinted polymer , solid phase extraction , metabolite , chromatography , chemistry , extraction (chemistry) , molecular imprinting , organic chemistry , biochemistry , selectivity , catalysis
A molecularly imprinted polymer (MIP) was synthesized in order to specifically extract vinflunine, an anticancer agent, and its metabolite (4‐ O ‐deacetylvinflunine) from bovine plasma and artificial urine by solid‐phase extraction (SPE). Vinorelbine, a non‐fluorinated analogue of vinflunine, was selected as a template for MIP synthesis. The selectivity of MIP versus the template (vinorelbine) and other alkaloids (catharanthine, vinblastine, vincristine, vinflunine and 4‐ O ‐deacetylvinflunine) was shown by a SPE protocol carried out with non‐aqueous samples. A second protocol was developed for aqueous samples with two consecutive washing steps (AcOH–NH 2 OH buffer (pH 7, I =10 mM)–MeOH mixture 95:5 v/v and ACN–AcOH mixture 99:1 v/v) and an elution step (MeOH–AcOH mixture 90:10 v/v). Thus, MIP‐SPE of bovine plasma brought high recoveries, 81 and 89% for vinflunine and its metabolite, respectively. This protocol was slightly modified for artificial urine samples in order to obtain a good MIP/NIP selectivity; furthermore, elution recoveries were 73 and 81% for vinflunine and its metabolite, respectively. Repeatability was assessed in both biological matrices and RSD (%) were inferior to 4%. The MIP also showed a suitable linearity ( r 2 superior to 0.99), between 0.25 and 10 μg/mL for plasma, and between 1 and 5 μg/mL for artificial urine.