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One‐step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross‐linked 12% agarose gel
Author(s) -
Liu Dan,
Ma Yan,
Wang Ye,
Su Zhiguo,
Gu Ming,
Janson JanChrister
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100006
Subject(s) - chemistry , chromatography , acetic acid , agarose , adsorption , ethanol , elution , tannin , organic chemistry , food science
The hydrolysable tannins corilagin and geraniin, the major active components of the traditional Chinese medicine Geranium wilfordii Maxim, have been separated and purified from crude extracts in one step by adsorption chromatography on cross‐linked 12% agarose gel (Superose 12 10/300 GL). The separation was achieved by gradient elution using mobile phase A composed of 5% ethanol and 5% acetic acid and mobile phase B composed of 30% ethanol and 30% acetic acid. The gradients were composed as follows: 0–240 mL, 0–25% B; 240–480 mL, 25–40% B; after 480 mL, 100% B. The purities of the collected corilagin and geraniin were 92.4 and 87.2%, and the corresponding yields were 88.0 and 76.8%, respectively.