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Development of a rapid and convenient method to separate eight ginsenosides from Panax ginseng by high‐speed counter‐current chromatography coupled with evaporative light scattering detection
Author(s) -
Shehzad Omer,
Jin Ha In,
Park Youmie,
Wan Ha Young,
Shik Kim Yeong
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000932
Subject(s) - chemistry , chromatography , chromatography detector , ginseng , countercurrent chromatography , elution , protopanaxadiol , mass spectrometry , ginsenoside , electrospray ionization , high performance liquid chromatography , medicine , alternative medicine , pathology
Ginsenosides exhibit diverse biological activities and are major well‐known components isolated from the radix of Panax ginseng C.A. Meyer. In the present work, a rapid and facile method for the separation and purification of eight ginsenosides from P. ginseng by high‐speed counter‐current chromatography coupled with evaporative light scattering detector (HSCCC‐ELSD) was successfully developed. The crude samples for HSCCC separation were first purified from ginseng extract using a macroporous resin; the extract was loaded onto a Diaion‐HP20 column and fractionated by methanol and water gradient elution. The ginsenosides‐protopanaxadiol (PPD) and protopanaxatriol (PPT) fractions were subsequently eluted with 65 and 80% methanol and water gradient elution, respectively. Furthermore, these two fractions were separated by HSCCC‐ELSD. The two‐phase solvent system used for separation was composed of chloroform/methanol/water/isopropanol at a volume ratio of 4:3:2:1. Each fraction obtained was collected and dried, yielding the following eight ginsenosides: Rg 1 , Re, Rf, Rh 1 , Rb 1 , Rc Rb 2 and Rd. The purity of these ginsenosides was greater than 97% as assessed by HPLC‐ELSD, and their structures were characterized by electrospray‐ionization mass spectrometry (ESI‐MS) and nuclear magnetic resonance spectroscopy. This is the first report regarding the separation of the ginsenosides Rh 1 , Rb 2 and Rc from P. ginseng by HSCCC.