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Magnetic mixed hemimicelles solid‐phase extraction of xanthohumol in beer coupled with high‐performance liquid chromatography determination
Author(s) -
Ding Jie,
Zhao Qi,
Sun Lei,
Ding Lan,
Ren Nanqi
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000930
Subject(s) - xanthohumol , chromatography , chemistry , extraction (chemistry) , detection limit , solid phase extraction , desorption , high performance liquid chromatography , bromide , adsorption , organic chemistry , ecology , key (lock) , biology
In this study, silica‐coated magnetic nanoparticles (Fe 3 O 4 /SiO 2 NPs) modified by cetyltrimethylammonium bromide (CTAB) were synthesized. They were successfully applied for extraction of xanthohumol in beer based on magnetic mixed hemimicelles solid‐phase extraction (MMHSPE) coupled with high‐performance liquid chromatography – ultraviolet determination. The main factors influencing the extraction efficiency including the surfactant amount, the beer pH, the extraction time, the desorption condition and the maximum extraction beer volume were optimized. Under the optimized conditions, a concentration factor of 60 was achieved by extracting 120 mL beer sample using MMHSPE and the detection limit of xanthohumol is 0.0006 mg/L. The proposed method was successfully applied for determination of xanthohumol in various beer samples with the xanthohumol contents in the range of 0.031–0.567 mg/L. The satisfactory recoveries (90–103%) were obtained in analyzing spiked beer samples.