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Hollow fiber‐based liquid–liquid–liquid microextraction followed by flow injection analysis using column‐less HPLC for the determination of phenazopyridine in plasma and urine
Author(s) -
Saraji Mohammad,
Bidgoli Ali Akbar Hajialiakbari,
Farajmand Bahman
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000929
Subject(s) - chromatography , extraction (chemistry) , detection limit , high performance liquid chromatography , chemistry , chromatography detector , fiber , analytical chemistry (journal) , volumetric flow rate , physics , organic chemistry , quantum mechanics
Hollow fiber‐based liquid–liquid–liquid microextraction (HF‐LLLME) followed by flow injection analysis and diode array detection (FIA‐DAD) was applied as a simple and sensitive quantitative method for the determination of phenazopyridine in urine and plasma samples. Flow injection system included a conventional HPLC system (without a chromatographic column) and a diode array detector. The extraction of phenazopyridine was carried out using diphenyl ether as the organic phase for filling the pores of the hollow fiber wall, and 0.1 M H 2 SO 4 solution as acceptor phase in the lumen of the fiber. The factors affecting the HF‐LLLME and flow injection analysis including type of organic solvent, pH of donor phase, extraction temperature, extraction time, stirring rate, and pH of mobile phase were investigated and the optimal extraction conditions were established. With the consumption of 5 mL of sample solution, the enrichment factor was about 230. The limit of detection was 0.5 μg/L with inter‐ and intra‐day precision being (RSD%) 6.9 and 4.9, respectively. Excellent linearity was found between 5 and 200 μg/L.