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An insight into the mechanism of CEC separation of template analogues on a norepinephrine‐imprinted monolith
Author(s) -
Huang BaoYu,
Chen YuCheng,
Liu ChuenYing
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000918
Subject(s) - monolith , itaconic acid , chemistry , molecularly imprinted polymer , divinylbenzene , chromatography , selectivity , ethylene glycol , monomer , monolithic hplc column , enantiomer , ethylene glycol dimethacrylate , copolymer , polymer , organic chemistry , high performance liquid chromatography , styrene , methacrylic acid , catalysis
A monolith molecularly imprinted polymer (MIP) column was prepared from template (−)‐norepinephrine, functional monomer (itaconic acid), and a cross‐linker (either ethylene glycol dimethacrylate or divinylbenzene) in porogen N,N ‐dimethylformamide. Understanding the molecular recognition of a template using an MIP seems feasible. However, it is hard to explain the recognition properties of their analogues on an MIP. The separation mechanism was investigated with the addition of charged surfactants, native and derivatised β‐cyclodextrin (β‐CD), achiral crown ether, etc. to determine the retention behaviour of the template analogues. The addition of organic modifiers and the adjustment of separation conditions were used to manipulate the selectivity. No chiral recognition was observed under most of the test conditions except the experiment with the charged β‐CD on the divinylbenzene‐MIP column. The different experimental conditions led to differences in the mobilities of the analytes and resulted in remarkable enantiomeric separation of the template. We confirmed the presence of mixed‐mode selectivity of the stationary phase based on hydrogen bonding, hydroelectric and hydrophobic interactions, and the electrophoretic mode.