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Comparative analysis of radical scavenging and antioxidant activity of phenolic compounds present in everyday use spice plants by means of spectrophotometric and chromatographic methods
Author(s) -
Stankevičius Mantas,
Akuņeca Ieva,
Jãkobsone Ida,
Maruška Audrius
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000915
Subject(s) - chemistry , dpph , apium graveolens , chromatography , antioxidant , scavenging , allium , high performance liquid chromatography , organic chemistry , botany , biology
Comparative analysis of radical scavenging and antioxidant activities of phenolic compounds present in everyday use spice plants was carried out by means of spectrophotometric and chromatographic methods. Six spice plant samples, namely onion ( Allium cepa ), parsley ( Petroselinum crispum ) roots and leaves, celery ( Apium graveolens ) roots and leaves and leaves of dill ( Anethum graveolens ) were analyzed. Total amount of phenolic compounds and radical scavenging activity (RSA) was the highest in celery leaves and dill extracts and was the lowest in celery roots. Comparing commonly used spectrophotometric analysis of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) RSA of extracts with the results obtained using reversed‐phase chromatographic separation with on‐line post‐column radical scavenging reaction detection, good correlation was obtained ( R 2 =0.848). Studies using HPLC system with electrochemical detector showed that bioactive phytochemicals can be separated and antioxidant activities of individual compounds evaluated without the need of a complex HPLC system with reaction detector. The results obtained using electrochemical detection correlate with the RSA assayed using spectrophotometric method ( R 2 =0.893).