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Fluorinated monolithic column for CEC
Author(s) -
Choodum Aree,
Smith Norman William,
Thavarungkul Panote,
Kanatharana Proespichaya
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000873
Subject(s) - monolith , monolithic hplc column , chemistry , monomer , cationic polymerization , dodecanol , chromatography , copolymer , acrylate , acrylic acid , solvent , styrene , polymer chemistry , organic chemistry , high performance liquid chromatography , polymer , catalysis
In order to overcome the drawbacks associated with particle‐based fluorinated stationary phases, a fluorinated cationic monolith was successfully synthesized by the in situ copolymerization of a 45% monomer mixture and 55% porogens with thermal initiation using 0.1% initiator with respect to the monomer mixture. The monomer mixture was composed of perfluorododecyl acrylate (49.75%), ethylene dimethacrylate (49.75%), and 0.5% of the quaternary amine acrylic monomer supporting an anodal electroosmotic flow. A tertiary porogenic solvent system consisting of 40% 1‐propanol, 40% 1‐octanol, and 20% 1‐dodecanol was used. The resulting monolith column showed good characteristics in terms of morphology, permeability (pressure drop was 4.8–25.7 MPa between 5 and 25 μL/min), and the electroosmotic mobility (3.68 (±0.05)×10 −4 cm 2 v −1 s −1 ). The monolith was tested for the separation of four phenolic compounds, three aromatic hydrocarbons, and one fluorine‐containing compound. Good efficiencies (up to 135 000 (±4000) plate/m and resolution up to 2.6 (±0.1)) with short analysis time (< 9 min for tau ‐fluvalinate, <7 min for four phenolic compounds, and <12 min for three aromatic hydrocarbons) and good stability (1.0–1.5% RSD for intraday, 1.2–1.6% RSD for interday, and 3.9–5.0% RSD for column‐to‐column) were obtained.