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Comprehensive two‐dimensional liquid chromatography with evaporative light‐scattering detection for the analysis of triacylglycerols in Borago officinalis
Author(s) -
Mondello Luigi,
Beccaria Marco,
Donato Paola,
Cacciola Francesco,
Dugo Giovanni,
Dugo Paola
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000843
Subject(s) - chromatography , chemistry , chromatography detector , selectivity , analytical chemistry (journal) , officinalis , high performance liquid chromatography , light scattering , aqueous solution , scattering , organic chemistry , optics , linguistics , philosophy , catalysis , physics
An optimized 2‐D liquid chromatography (LC×LC) set‐up, based on the different selectivities of a silver ion (Ag) and a non‐aqueous reversed phase (NARP), employed in the first (D1) and the second dimension (D2), respectively, in combination with evaporative light‐scattering detection (ELSD), has been developed for the analysis of the triacylglycerol (TAG) fraction in a Borago officinalis oil. The 2‐D set‐up, thanks to the complementary separation selectivity provided by the two columns, allowed to distribute 78 TAGs throughout the 2‐D LC retention plane otherwise unachievable by 1‐D LC.