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Time‐varying migration process of moving neutralization boundary on the immobilized pH gradient strip in the weak‐base rehydration buffer
Author(s) -
Liang Heng,
OuYang LiangFei,
Liu Qian,
Zhang Lin,
Tian LiJing,
Chen Yang
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000813
Subject(s) - dissociation (chemistry) , ammonia , chemistry , neutralization , recursion (computer science) , chromatography , analytical chemistry (journal) , concentration gradient , computer science , algorithm , organic chemistry , antibody , immunology , biology
This paper quantificationally probes into time‐varying migration processes of moving neutralization boundary (MNB) on immobilized pH gradient (IPG) strip in ammonia‐rehydration buffers. The time‐varying migration processes are determined by both time‐varying dissociation equilibria of ammonia and position‐varying pH environments formed by immobilized carrier ampholytes (CAs) on the IPG strip. Thus, the local dissociation equilibria of ammonia and the position‐varying pH are introduced into the recursion equation of position of MNB migrations. The theoretical position–time curves and the velocity–time curves of MNB migrations obtained by the recursion approach were satisfactorily validated by a series of images of boundary migrations from the IPG‐MNB experiments by using rehydration buffers with different ammonia concentrations on pH 3–6 IPG strips. The results achieved herein have significant evidence to a quantificational understanding of the mechanism of MNB and IEF.