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Determination of bavachin and isobavachalcone in Fructus Psoraleae by high‐performance liquid chromatography with electrochemical detection
Author(s) -
Li Yuan,
Wang Fang,
Chen Zilin
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000801
Subject(s) - chromatography , detection limit , chemistry , analyte , high performance liquid chromatography , acetonitrile , relative standard deviation , analytical chemistry (journal) , correlation coefficient , linear regression , mathematics , statistics
A simple, sensitive and selective method of high‐performance liquid chromatography with electrochemical detection (HPLC‐ECD) has been developed for simultaneous determination of bavachin and isobavachalcone in Fructus Psoraleae . At optimized conditions, bavachin and isobavachalcone could be well separated within 15 min at a detection potential of +0.80 V with 0.03 mol/L acetate buffer solution (pH 5.17)/acetonitrile (2:3, v/v) as the mobile phase. The relationships between peak areas and concentrations were linear from 8.26×10 −7 to 1.21×10 −4 mol/L for bavachin, and from 1.01×10 −8 to 1.61×10 −4 mol/L for isobavachalcone, respectively. The method offered excellent linearity with regression coefficient R 2 >0.995. The method presented detection limits ( S / N =3) of 8.81×10 −9 mol/L for bavachin and 1.17×10 −10 mol/L for isobavachalcone. It indicates that the sensitivity of electrochemical detection is ten times higher than that of diode array detection (DAD). The mean recoveries around 98% with a relative standard deviation less than 3.1% for the two analytes have been obtained. The proposed separation and detection procedures were successfully applied to the simultaneous determination of bavachin and isobavachalcone in traditional Chinese medicine.