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A highly efficient and versatile microchip capillary electrophoresis method for DNA separation using gold nanoparticle as a tag
Author(s) -
Cao Wei,
Chen Lei,
Fu Youjian,
Tan Zhenyi,
Qu Bin
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000755
Subject(s) - capillary electrophoresis , horseradish peroxidase , electrophoresis , chemistry , chromatography , colloidal gold , dna , gel electrophoresis , nanoparticle , combinatorial chemistry , nanotechnology , materials science , biochemistry , enzyme
A highly efficient and versatile method for DNA separation using Au nanoparticles (Au NPs) as a tag based on microchip capillary electrophoresis (MCE) was developed. The thiol‐modified DNA‐binding Au NPs were utilized as a tag. Target DNA was sandwiched between Au NPs and probe DNA labeled with horseradish peroxidase (HRP). In electrophoresis separation, the difference in electrophoretic mobility between free probe and probe‐target complex was magnified by Au NPs, which enabled the resulting mixture to be separated with high efficiency by microchip capillary electrophoresis. Horseradish peroxidase was used as a catalytic label to achieve sensitive electrochemical DNA detection via fast catalytic reactions. With this protocol, 27‐mer DNA fragments with different sequences were separated with high speed and high resolution. The proposed method was critical to achieve improved DNA separations in hybridization analyses.