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Retention prediction in reversed‐phase liquid chromatography systems with methanol/water mobile phases containing different alkanols as additives
Author(s) -
PappaLouisi Adriani,
Agrafiotou Panagiota,
Fasoula Stella
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000725
Subject(s) - alkylbenzenes , chemistry , methanol , phase (matter) , chromatography , alcohol , solvent , alkyl , selectivity , propanol , high performance liquid chromatography , kovats retention index , butanol , ethanol , organic chemistry , gas chromatography , benzene , catalysis
In an effort to gain enhancement of selectivity in reversed‐phase liquid chromatography, retention was tuned in this study by introducing short and medium straight‐chained‐length alkanol additives (methanol (MeOH), ethanol, 1‐propanol, 1‐butanol, 1‐pentanol, 1‐hexanol or 1‐heptanol) at low concentrations in mobile phases containing MeOH as the main organic solvent. A six‐parameter retention model considering simultaneously the contents of the main organic modifier and of the alcohol additive as well as of the number of alkyl chain of additive was developed by a direct combination of equations expressing separately a linear dependence of the retention upon each of these factors. The effectiveness of the above model was tested in the retention prediction of a mixture of six alkylbenzenes under isocratic conditions with mobile phases containing as an additive any member of the homologues series of alkanols (with 1–7 carbon atoms) at different low concentrations in a wide range of MeOH–water mixtures. The prediction was excellent in all cases even when the alkanol additives used in experiments for the fitting procedure are different than those used in chromatographic runs done for testing the prediction ability of the proposed model.