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Separation of γ‐linolenic and other polyunsaturated fatty acids from Boraginaceae via supercritical CO 2
Author(s) -
Ghoreishi S. M.,
Mardani E.,
Ghaziaskar H. S.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000716
Subject(s) - boraginaceae , chromatography , supercritical fluid , extraction (chemistry) , flame ionization detector , supercritical carbon dioxide , linolenic acid , chemistry , supercritical fluid extraction , gas chromatography , oleic acid , linoleic acid , fatty acid , organic chemistry , botany , biochemistry , biology
Essential fatty acids were extracted from Echium amoenum (Boraginaceae) seed oil via supercritical carbon dioxide and the results were compared with conventional Soxhlet method as the base case of 100% recovery. The response surface methodology was used to optimize the effective extraction parameters. The chemical composition of recovered oil was analyzed by polar and non‐polar gas chromatograph‐flame ionization detector columns. The experimental results indicated that echium seed contained 25 wt% oil and the maximum extraction oil recovery of 92% was obtained via supercritical CO 2 at optimal operating conditions (43°C, 280 bar, 1.5 mL/min, 25 min static time and 130 min dynamic time). At similar operating conditions, applying 2.5 and 5 mol% ethanol as a modifier enhanced the recovery to 96 and 112%, respectively. The results showed that supercritical fluid extraction is a viable technique for separation of constituents such as γ‐linolenic acid (7–8%), palmitic acid (6–7%), stearic acid (3–4%), oleic acid (12–13%), linoleic acid (19–20%), α‐linolenic acid (40–41%) and stearidonic acid (8–9%) from Boraginaceae.