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Chitosan–silica hybrid‐coated open tubular column for hydrophilic interaction capillary electrochromatography
Author(s) -
Hu Jinmei,
Yin Lili,
Jia Li
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000688
Subject(s) - capillary electrochromatography , chemistry , chitosan , covalent bond , chromatography , capillary electrophoresis , silane , monolithic hplc column , amine gas treating , selectivity , electrochromatography , electrophoresis , capillary action , chemical engineering , high performance liquid chromatography , organic chemistry , materials science , catalysis , composite material , engineering
A novel and convenient protocol for the preparation of an open‐tubular column coated with chitosan–silica hybrid using chitosan and silane‐coupling agent (γ‐glycidoxy‐propyltrimethoxysilane) was developed for CEC, in which, chitosan was covalently bonded to the inner wall of a fused‐silica capillary using γ‐glycidoxy‐propyltrimethoxysilane as a cross‐linking agent. The stationary phase was hydrophilic due to the chitosan–silica hybrid with abundant amine and hydroxyl functional groups. The chromatographic characteristics of the column were evaluated by the separation of some organic acids and inorganic anions. The column showed good selectivity for nucleotides, aromatic acids, and inorganic anions. The mechanism for the separation of these compounds was primarily based on the hydrophilic and electrostatic interactions combined with the electrophoretic mechanism. The CEC method on the column for the separation of these compounds was compared with CE method in a bare capillary.