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Liquid chromatography/mass spectrometry‐based metabolic profiling to elucidate chemical differences of tobacco leaves between Zimbabwe and China
Author(s) -
Li Qinghua,
Zhao Chunxia,
Li Yong,
Chang Yuwei,
Wu Zeming,
Pang Tao,
Lu Xin,
Wu Yi,
Xu Guowang
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000652
Subject(s) - chromatography , metabolomics , mass spectrometry , chemistry , metabolite profiling , repeatability , metabolite , liquid chromatography–mass spectrometry , biochemistry
An approach was developed for extracting and analyzing the chemical components of tobacco leaves based on solvent extraction and rapid & resolution liquid chromatography/quadrupole time‐of‐flight mass spectrometry analysis. Two solvents with different polarities were used to extract hydrophilic components and hydrophobic components, respectively, the combined analytical data can provide a “global” view of metabolites. Based on the evaluation of parallel samples, it was found that this approach provided good repeatability, accurate and reliable profiling data, and is suitable for the metabolomics study of tobacco leaves. In order to find the chemical component differences of tobacco leaves, 56 samples from Zimbabwe and China were analyzed using the developed method. The metabolite data were processed by multivariate statistic technique; an obvious group classification between Zimbabwe and China was observed, 14 significantly changed compounds were found, and 9 of them were identified.