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Simultaneous separation and identification of limonoids from citrus using liquid chromatography‐collision‐induced dissociation mass spectra
Author(s) -
Jayaprakasha Guddadarangavvanahally K.,
Dandekar Deepak V.,
Tichy Shane E.,
Patil Bhimanagouda S.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000644
Subject(s) - limonin , chemistry , chromatography , mass spectrum , glucoside , mass spectrometry , high performance liquid chromatography , fragmentation (computing) , collision induced dissociation , dissociation (chemistry) , tandem mass spectrometry , organic chemistry , medicine , alternative medicine , pathology , computer science , operating system
Abstract Limonoids are considered as potential cancer chemopreventive agents and are widely distributed in the Citrus genus as aglycones and glucosides. In the present study, reversed‐phase HPLC coupled with CID mass spectra was developed for the simultaneous separation and identification of aglycones and glucosides of limonoids from citrus. Five aglycones such as limonin, deacetyl nomilin, ichangin, isolimonoic acid and nomilin were identified by positive ion CID MS/MS, whereas five glucosides, viz. limonin glucoside, isoobacunoic acid glucoside, obacunone glucoside, deacetyl nomilinic acid glucoside and nomilinic acid glucoside were analyzed by negative ion CID mass spectra. The developed method was successfully applied to complex citrus samples for the separation and identification of aglycones and glucosides. Citrus seeds were extracted with methanol and partially purified and analyzed by LC‐CID mass spectra. The separation was achieved by C‐18 column; eight limonoids were identified by comparing the retention times and mass spectral fragmentation. To the best of our knowledge, this is the first report on the identification of citrus limonoids using CID technique.