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Preparation and characterization of magnetic chitosan microsphere sorbent for separation and determination of environmental estrogens through SPE coupled with HPLC
Author(s) -
Xu Zhixiang,
Zhang Jing,
Cong Li,
Meng Ling,
Song Jiaming,
Zhou Jie,
Qiao Xuguang
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000640
Subject(s) - sorbent , chromatography , adsorption , detection limit , chitosan , chemistry , glutaraldehyde , high performance liquid chromatography , magnetic nanoparticles , desorption , diethylstilbestrol , nanoparticle , materials science , nanotechnology , biochemistry , organic chemistry , hormone
In this paper, a new separation sorbent of magnetic chitosan microsphere was prepared by means of suspension process using magnetic‐Fe 3 O 4 nanoparticles as magnetic material and glutaraldehyde as the cross‐linker. The morphology and properties of magnetic chitosan microspheres were characterized by FT‐IR, SEM and adsorption test, and the results showed that they had good recognition, high selective ability and fast adsorption–desorption dynamics for estriol (E3), 17β‐estradiol (E2) and diethylstilbestrol (DES). Applying this material as SPE sorbent, a method for separation, enrichment and analysis of estrogens in samples coupled with HPLC was developed. Under the optimized condition, the detection limit ( S / N =3) for three estrogens was 3.2–20.1 ng/L, and the RSD for five replicate extractions of 50 μg/L E3, E2 and DES was 4.6–8.3%. The blank river water spiked with E3, E2 and DES at 0.05 and 0.2 mg/L levels were extracted and determined by the developed method with good recoveries ranging from 85.5 to 103.7%.