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Separation and determination of β‐casomorphins by using glass microfluidic chip electrophoresis together with laser‐induced fluorescence detection
Author(s) -
Wang ZongWen,
Wang WenJian,
Wang Wei,
Xu LiangJun,
Chen GuoNan,
Fu FengFu
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000634
Subject(s) - fluorophore , chromatography , detection limit , laser induced fluorescence , microfluidics , derivatization , fluorescence , microfluidic chip , reagent , chemistry , capillary electrophoresis , analytical chemistry (journal) , electrophoresis , excitation wavelength , materials science , mass spectrometry , nanotechnology , optics , physics
A simple, reliable and reproducible method for the separation and determination of five β‐casomorphins (β‐CMs, namely TPGN, PGPI, TPGI, TPGP and TPPG) based on glass microfluidic chip electrophoresis and laser‐induced fluorescence detection is first described in here. The microfluidic chip electrophoresis and laser‐induced fluorescence detection system consisted of a home‐made glass “double‐T” microchip and a simple LIF detector with excitation and emission wavelengths of 473 and 525 nm, respectively. Fluorescein isothiocyanate (FITC) was used as the precolumn derivatization reagent to label fluorophore on five β‐CMs, and the optimum conditions of FITC‐derivatization reaction and MCE separation were investigated in detail. Under optimum conditions, five β‐CMs were completely separated and detected within 30 min with a detection limit of 18.7–75.1 nmol/L and an RSD ( n =5) of 3.0–5.9%, respectively. The proposed method has been successfully used to detect β‐CMs in real cheese sample with a recovery of 89–109%, suggesting that our method is sensitive and reliable. These features, as well as its low cost, operation convenience, stability and reusability, make it a promising alternative to β‐CMs detection methods.