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Proteome profile of zebrafish caudal fin based on one‐dimensional gel electrophoresis LCMS/MS and two‐dimensional gel electrophoresis MALDI MS/MS analysis
Author(s) -
Singh Sachin K.,
Meena Lakshmi Mula G.,
Saxena Sandeep,
Swamy Cherukuvada V. Brahmendra,
Idris Mohammed M.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000626
Subject(s) - zebrafish , proteome , two dimensional gel electrophoresis , gel electrophoresis , biology , polyacrylamide gel electrophoresis , proteomics , microbiology and biotechnology , chemistry , biochemistry , enzyme , gene
Abstract Zebrafish ( Danio rerio ) is the widely used vertebrate model animal for understanding the complexity of development and disease process. Zebrafish has been also extensively used in understanding the mechanism of regeneration for its extensive capability of regenerating fins and other tissues. We have analyzed the proteome profile of zebrafish caudal fin in its native state based on one‐dimensional gel electrophoresis LCMS/MS and two‐dimensional gel electrophoresis MS/MS analyses. A total of 417 proteins were identified as zebrafish fin tissue specific, which includes 397 proteins identified based on one‐dimensional gel electrophoresis LCMS/MS analysis and 101 proteins identified based on two‐dimensional gel electrophoresis MALDI MS/MS. The proteins mapped to the zebrafish fin tissue were shown to be involved in various biological activities related to development, apoptosis, signaling and metabolic process. Focal adhesion, regulation of actin cytoskeleton, cancer‐related pathways, mitogen‐activated protein kinase signaling, antigen processing and presentation, and proteasome are some of the important pathways associated with the identified proteome data set of the zebrafish fin.