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Ultra‐performance LC‐photodiode array‐eλ‐ESI‐MS/MS screening method for the detection of radical‐scavenging natural antioxidants from Radix et Rhizoma Rhei
Author(s) -
Wang GuoYing,
Chen FangFang,
Shi YanPing
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000594
Subject(s) - chemistry , gallic acid , radix (gastropod) , chromatography , emodin , antioxidant , scavenging , glycoside , chemiluminescence , reactivity (psychology) , stereochemistry , organic chemistry , botany , biology , medicine , alternative medicine , pathology
A novel ultra‐performance LC‐photodiode array‐eλ‐ESI‐MS/MS screening method was developed for the detection and identification of natural antioxidants from Radix et Rhizoma Rhei . Nine compounds were found to possess a potential antioxidant activity, and their free radical‐scavenging capacities were investigated in detail. The nine compounds were identified as 1‐ O ‐galloyl‐2‐ O ‐cinnamoylglucose, 6‐hydroxymusizin‐8‐ O ‐β‐ D ‐glucopyranoside, (+)‐catechin, gallic acid 3‐ O ‐β‐ D ‐glucopyranoside, trans‐3,5,4′‐trihydroxystilbene‐4′‐ O ‐β‐ D ‐(2″‐ O ‐galloyl)‐glucopyranoside, sennoside A, 4‐(4′‐hydroxyphenyl)‐2‐butanone‐4′‐ O ‐β‐ D ‐(2″‐ O ‐galloyl‐6″‐ O ‐ p ‐coumaroyl) glucopyranoside, emodin‐8‐ O ‐(6′‐ O ‐malonyl) glucopyranoside, and physcion‐8‐ O ‐β‐ D ‐glucopyranoside. The reactivity and SC 50 values of those compounds were investigated, respectively. 1‐ O ‐Galloyl‐2‐ O ‐cinnamoylglucose showed the strongest capability for scavenging 1,1‐diphenyl‐2‐picrylhydrazyl‐free radical; trans‐3,5,4′‐trihydroxystilbene‐4′‐ O ‐β‐ D ‐(2″‐ O ‐galloyl) glucopyranoside showed the strongest capability for scavenging superoxide radical; 4‐(4′‐hydroxyphenyl)‐2‐butanone‐4′‐ O ‐2‐ D ‐(2″‐ O ‐galloyl‐6″‐ O ‐ p ‐coumaroyl) glucopyranoside exhibited the highest reactivity in the lipid peroxidation processes. The use of the analytical screening method based on ultra‐performance LC‐photodiode array‐eλ‐ESI‐MS/MS would provide a new way for rapid detection of radical‐scavenging natural compounds from Radix et Rhizoma Rhei or complex matrices.