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Determination of analytes in medical herbs extracts by SPE coupled with two‐dimensional planar chromatography in combination with diode array scanning densitometry and HPLC‐diode array detector
Author(s) -
Tuzimski Tomasz
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000582
Subject(s) - chromatography , chromatography detector , high performance liquid chromatography , analyte , solid phase extraction , chemistry , elution , detection limit
The purpose of this study is to demonstrate an application of 2‐D high‐performance planar chromatography‐diode array detector (DAD) and HPLC‐DAD after solid‐phase extraction (SPE) for identification and quantitative analysis of pesticides (isoproturon, aziprotryne, hexazinone, flufenoxuron, methabenzthiazuron, procymidone, and α‐cypermethrin) in Melissa officinalis L. (Labiatae) samples. The procedure described for the determination of compounds is inexpensive and can be applied to routine analysis of analytes in medical herbs' samples after preliminary cleanup and concentration by SPE. Average recoveries on C18 SPE cartridges of pesticides eluted with 5 mL tetrahydrofuran by the proposed HPLC‐DAD method, before and after 2‐D‐high‐performance planar chromatography separation of analytes from M. officinalis L. samples spiked with pesticide at a concentration level of 10 μg/g in plant material are presented. Method validation parameters for the quantification of pesticides by the proposed HPLC‐DAD after SPE method are also presented.