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Simultaneous determination of methylxanthines in different types of tea by a newly developed and validated TLC method
Author(s) -
Cimpoiu Claudia,
Hosu Anamaria,
Seserman Liana,
Sandru Mihaela,
Miclaus Vasile
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000554
Subject(s) - chromatography , theobromine , chemistry , maceration (sewage) , silica gel , dichloromethane , caffeine , theophylline , solvent , materials science , medicine , organic chemistry , composite material , endocrinology
The aim of this paper is to develop a new simple, fast and economical method for simultaneous quantitative determination of methylxanthine compounds based on TLC combined with image analysis. To obtain certain results, both extraction and chromatographic separation were optimized. The optimum extraction conditions were maceration in ethanol–water 8:2, v/v. The chromatographic separations were done on the silica gel F 254 TLC plates developed with chloroform–dichloromethane–isopropanol, 4:2:1 v/v/v. Detection was performed under UV lamp at 254 nm and the evaluation of the chromatographic plate was based on digital processing of chromatographic images. The developed TLC method was validated for parameters such as specificity, linearity and range, LOD and LOQ, precision, robustness and accuracy. This method was then applied for determination of caffeine, theobromine and theophylline in different types of tea, commercially available. Moreover, the content of methylxanthines detected and determined in commercial tea samples can be used as chemical marker in quality control.