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Micron‐sized pillars for ion‐pair reversed‐phase DNA separations
Author(s) -
De Malsche Wim,
Zhang Lei,
Op De Beeck Jeff,
Vangelooven Joris,
Majeed Bivragh,
Desmet Gert
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000538
Subject(s) - dna , phase (matter) , chemistry , ion , chromatography , analytical chemistry (journal) , biochemistry , organic chemistry
In the present paper, the feasibility to construct micron‐sized silicon pillar channels to be used in HPLC is studied. For this, a channel with flow‐through pores of 1 μm and with critical sidewall dimensions below 1 μm was constructed using advanced deep‐UV lithographic equipment. Integrating a 3‐nL injection system on the chip directly in front of the separation channel and using elongated distribution structures, a very controlled and high aspect ratio sample definition across the relatively wide separation channel was accomplished. The system was evaluated in isocratic ion‐pair RP mode, allowing the separation of a mixture of two components with, respectively, 300 and 400 base pairs in 5 s only.