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Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal‐phase liquid chromatography fractionation with reversed‐phase liquid chromatography‐mass spectrometry
Author(s) -
Ding Li,
Xie Fuwei,
Xu Guowang,
Liu Kejian,
Wang Sheng,
Xie Jianping
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000536
Subject(s) - chromatography , fractionation , chemistry , fructose , mass spectrometry , reversed phase chromatography , high performance liquid chromatography , polar , biochemistry , physics , astronomy
Integration of normal‐phase LC (NPLC) fractionation with RPLC‐ESI/MS was established to detect the polar fractions of cuticular components from Oriental tobacco leaf. NPLC was selected for the fractionation of polar components of cuticular leaf extract, after being concentrated with rotary evaporator, each of the enriched fractions was further analyzed by RPLC‐ESI/MS. In total, 83 compounds were finally detected including 45 cembranoids, 15 labdanoids, 20 sucrose esters, and 3 glucose esters (or fructose esters). Three cembranoids and seven labdanoids possibly are new diterpenoids. Glucose esters (or fructose esters) are also reported in Nicotiana tobacco for the first time .