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Reversed‐phase HPLC of peptides: Assessing column and solvent selectivity on standard, polar‐embedded and polar endcapped columns
Author(s) -
Mant Colin T.,
Cepeniene Dziuleta,
Hodges Robert S.
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000518
Subject(s) - chemistry , selectivity , alkyl , peptide , chromatography , polar , amide , reversed phase chromatography , side chain , high performance liquid chromatography , structural isomer , hydrophilic interaction chromatography , aqueous solution , ether , solvent , combinatorial chemistry , organic chemistry , polymer , biochemistry , physics , astronomy , catalysis
We desired to evaluate the chromatographic selectivity for peptides of silica‐based RP high‐performance liquid chromatography stationary phases with various modifications (polar embedding and polar endcapping on C 18 columns; ether‐linked phenyl column with polar endcapping) compared with n ‐alkyl (C 18 , C 8 ) and aromatic phenylhexyl columns. Thus, we have designed and synthesized two series of synthetic peptide standards with the sequence Gly–Gly–Leu–Gly–Gly–Ala–Leu–Gly– X –Leu–Lys–Lys–amide, where the N‐terminal either contains a free α‐amino group (AmC series) or is N α ‐acetylated (AcC series) and where position X is substituted by Gly, Ala, Val, Ile, Phe or Tyr. These represent series of peptides with single substitutions of n ‐alkyl (Gly