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Optimisation of ultra‐performance LC conditions using response surface methodology for rapid separation and quantitative determination of phenolic compounds in Artemisia minor
Author(s) -
Zhou Yan,
FungKei Choi Franky,
He Zhi Zhou,
Song JingZheng,
Qiao ChunFeng,
Liu Xin,
Ding LiSheng,
Gesang SuoLang,
Xu HongXi
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000452
Subject(s) - chromatography , high performance liquid chromatography , chemistry , response surface methodology , resolution (logic) , gradient elution , artificial intelligence , computer science
A method that couples rapid, sensitive, reproducible and accurate ultra‐performance LC (UPLC) with quadrupole‐TOF‐MS was established for the first simultaneous qualitative and quantitative analysis of phenolic compounds in Artemisia minor . Box–Behnken designs (BBDs) were applied as an effective tool to optimise major parameters that influence the resolution of UPLC, including three gradient steps and column temperature. Under optimal UPLC conditions, a total of 23 phenolic compounds in the crude methanol extracts of A. minor were well separated on a Waters Acquity UPLC BEH C 18 column (100×2.1 mm, 1.7 μm particle size) within 16.5 min, and the compounds were unequivocally or tentatively identified via comparisons with authentic standards and literature. In this study, a total of six major phenolic compounds were quantified in A. minor and the method was validated to be sensitive, precise and accurate within the LOD from 1.24 to 5.27 μg/mL, and the overall intra‐ and inter‐day variations in detection were less than 3.76%. The recovery of the method ranged from 97.9 to 103.8% with RSDs that were less than 5.8%. These results demonstrate that this approach has the potential for quality control of A. minor and other Tibetan herbal medicines.