Premium
Surface‐modified TiO 2 nanoparticles as affinity probes and as matrices for the rapid analysis of phosphopeptides and proteins in MALDI‐TOF‐MS
Author(s) -
Ke Yaotang,
Kailasa Suresh Kumar,
Wu HuiFen,
Nawaz Mohd
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000415
Subject(s) - chemistry , nanoparticle , mass spectrometry , chromatography , matrix assisted laser desorption/ionization , analytical chemistry (journal) , nanotechnology , adsorption , materials science , desorption , organic chemistry
We utilized three different types of TiO 2 nanoparticles (NPs) namely TiO 2 ‐dopamine, TiO 2 ‐CdS and bare TiO 2 NPs as multifunctional nanoprobes for the rapid enrichment of phosphopeptides from tryptic digests of α‐ and β‐casein, milk and egg white using a simplified procedure in MALDI‐TOF‐MS. Surface‐modified TiO 2 NPs serve as effective matrices for the analysis of peptides (gramicidin D, HW6, leucine‐enkephalin and methionine‐enkephalin) and proteins (cytochrome c and myoglobin) in MALDI‐TOF‐MS. In the surface‐modified TiO 2 NPs‐based MALDI mass spectra of these analytes (phosphopetides, peptides and proteins), we found that TiO 2 ‐dopamine and bare TiO 2 NPs provided an efficient platform for the selective and rapid enrichment of phosphopeptides and TiO 2 ‐CdS NPs efficiently acted as the matrix for background‐free detection of peptides and proteins with improved resolution in MALDI‐MS. We found that the upper detectable mass range is 17 000 Da using TiO 2 ‐CdS NPs as the matrix. The approach is simple and straightforward for the rapid analysis of phosphopeptides, peptides and proteins by MALDI‐MS in proteome research.