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Microwave‐assisted extraction and in situ clean‐up for the determination of fluoroquinolone antibiotics in chicken breast muscle by LC‐MS/MS
Author(s) -
Xu Haoyan,
Chen Ligang,
Sun Lei,
Sun Xin,
Du Xiaobo,
Wang Jian,
Wang Tianyi,
Zeng Qinglei,
Wang Hui,
Xu Yang,
Zhang Xiaopan,
Ding Lan
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000365
Subject(s) - fleroxacin , chromatography , chemistry , sparfloxacin , enrofloxacin , extraction (chemistry) , ciprofloxacin , sample preparation , tandem mass spectrometry , phosphoric acid , mass spectrometry , antibiotics , ofloxacin , biochemistry , organic chemistry
A new method was developed for the determination of six fluoroquinolone antibiotics including fleroxacin, levofloxacin, ciprofloxacin, lomfloxacin, enrofloxacin, and sparfloxacin in chicken breast muscle, in which the extraction and clean‐up were performed in one step by microwave irradiation. The mixture of ACN containing 0.3% v/v phosphoric acid/water pH 3 (70:30, v/v) was used as the extraction solution and hexane was used as the clean‐up solution. The extract was analyzed by liquid chromatography–tandem mass spectrometry system. The RSDs of intra‐ and inter‐day obtained are in the range of 1.0–10.4 and 3.8–13.6%, respectively. In the three fortified levels of chicken breast muscle (20, 100, and 500 ng/g), the recoveries of fluoroquinolone antibiotics ranging from 66.0 to 97.2% are obtained. The LODs are in the range of 2.7–6.7 ng/g. This method simplifies the process of the sample preparation and reduces the operation errors.