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Preparation of high‐selective HPLC packing materials based on alternating copolymer‐grafted silica
Author(s) -
Mallik Abul K.,
Sawada Tsuyoshi,
Takafuji Makoto,
Ihara Hirotaka
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000293
Subject(s) - copolymer , selectivity , acrylate , magic angle spinning , elution , chemistry , high performance liquid chromatography , fourier transform infrared spectroscopy , thermogravimetric analysis , styrene , vinyl acetate , diffuse reflectance infrared fourier transform , magic angle , monomer , materials science , chromatography , analytical chemistry (journal) , nuclear magnetic resonance spectroscopy , polymer , organic chemistry , chemical engineering , catalysis , photocatalysis , engineering
Three alternating copolymer‐grafted silica stationary phases for use in high‐selective RP‐HPLC were prepared from two vinyl monomers selected from styrene, N ‐methylmaleimide, N ‐octadecylmaleimide, and octadecyl acrylate; they were characterized by elemental analyses, thermogravimetric analysis, diffuse reflectance infrared Fourier transform spectroscopy, and 13 C cross‐polarization magic‐angle spinning NMR spectroscopy. Aspects of molecular‐shape selectivity were evaluated for three different columns using Standard Reference Material 869b, Column Selectivity Test Mixture for Liquid Chromatography. The best selectivity for isomer separations was obtained for the stationary phase prepared with a copolymer of octadecyl acrylate and N ‐octadecylmaleimide, which was able to separate 16 polycyclic aromatic hydrocarbons (Standard Reference Material 1647e) in an isocratic elution. In this paper, the effectiveness of this phase is also demonstrated by separation of tocopherol isomers.