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Methacrylate ester‐based monolithic columns for nano‐LC separation of tocopherols in vegetable oils
Author(s) -
LermaGarcía María Jesús,
Cerretani Lorenzo,
HerreroMartínez José Manuel,
Bendini Alessandra,
SimóAlfonso Ernesto Francisco
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000246
Subject(s) - repeatability , chromatography , chemistry , resolution (logic) , high performance liquid chromatography , methacrylate , vegetable oil , methanol , organic chemistry , polymer , copolymer , artificial intelligence , computer science
The separation and determination of tocopherols (Ts) in vegetable oils by nano‐LC chromatography with UV–vis detection using lauryl methacrylate ester‐based monolithic columns has been developed. The separation of Ts was optimized in terms of mobile phase composition on the basis of the best compromise among efficiency, resolution and analysis time. Using a mobile phase composed of ACN/methanol/water, an excellent resolution between Ts was achieved within 18 min. The LODs were lower than 0.26 μg/mL, being repeatability values of retention time and peak area below 0.15 and 3.1%, respectively. The method was applied to the quantification of Ts and tocotrienols present in several vegetable oils from different botanical origins.