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Development of the fingerprint for the quality of Radix Linderae through ultra‐pressure liquid chromatography‐photodiode array detection/electrospray ionization mass spectrometry
Author(s) -
Wu Yongjiang,
Zheng Yunliang,
Luan Lianjun,
Liu Xuesong,
Han Zheng,
Ren Yiping,
Gan Lishe,
Zhou Changxin
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000243
Subject(s) - radix (gastropod) , chromatography , fingerprint (computing) , mass spectrometry , correlation coefficient , analytical chemistry (journal) , chemistry , high performance liquid chromatography , atmospheric pressure chemical ionization , mass spectrum , ionization , chemical ionization , mathematics , statistics , artificial intelligence , botany , computer science , biology , ion , organic chemistry
An ultra‐pressure LC (UPLC) method has been developed and validated for the quality evaluation of a traditional Chinese medicine ( Radix Linderae ) by chemical fingerprint analysis with chromatograms collected at two wavelengths (260 and 320 nm). Eleven characteristic peaks in the fingerprints were identified by comparing their retention times, UV spectra and ESI‐MS/MS data with those of the reference substances or the data in the literatures. Both correlation coefficient of similarities in chromatograms and relative peak areas of common peaks were calculated for quality expression of the Radix Linderae samples collected from different areas in China. The results showed high variation of relative peak area and correlation coefficients among the samples collected from various habitats, which indicated that the quality consistency of Radix Linderae is still a problem worthy of serious concern.