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Preparation and characterization of ceria‐zirconia composite for enrichment and identification of phosphopeptides
Author(s) -
Wu JianHong,
Xiao Kuang,
Zhao Yong,
Zhang WeiPing,
Guo Lin,
Feng YuQi
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000224
Subject(s) - cubic zirconia , x ray photoelectron spectroscopy , materials science , composite number , adsorption , phosphoprotein , dephosphorylation , specific surface area , zirconium , chemical engineering , mass spectrometry , catalysis , chemistry , chromatography , composite material , organic chemistry , phosphatase , ceramic , biochemistry , phosphorylation , engineering , metallurgy , enzyme
Ceria‐zirconia composites at different molar ratios were synthesized. Several methods were used to characterize these composites, including X‐ray photoelectron spectroscopy, surface area and surface acid‐base property detection. A one‐step method for isolation and identification of phosphopeptides from peptide mixture was created using these ceria‐zirconia composites. Using tryptic digest of standard phosphorylated protein, we have shown that these enrichment and dephosphorylation activities are effective. The adsorption capacity and catalytic property of ceria‐zirconia composites at different molar ratios and calcinated temperatures were studied. In combination with MALDI‐TOF‐based peptide mass finger printing technique, we have established a method to utilize the enrichment/dephosphorylation dual properties of these ceria‐zirconia composites for the analysis of phosphoprotein in nonfat milk successfully.