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HPLC‐PAD‐atmospheric pressure chemical ionization‐MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea‐star)
Author(s) -
Ferreres Federico,
Pereira David M.,
GilIzquierdo Angel,
Valentão Patrícia,
Botelho João,
Mouga Teresa,
Andrade Paula B.
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000197
Subject(s) - astaxanthin , metabolite , lutein , atmospheric pressure chemical ionization , chemistry , carotenoid , high performance liquid chromatography , zeaxanthin , chromatography , botany , biochemistry , chemical ionization , biology , ionization , organic chemistry , ion
Abstract An HPLC‐PAD‐atmospheric pressure chemical ionization‐MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea‐star). Bio‐guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL‐2H3 (IC 25 =268 μg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC 25 =411 μg/mL)) cell line, however, toxicity was lower, as it is desired for anti‐cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.