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Simultaneous analysis of seven alkaloids in Coptis‐Evodia herb couple and Zuojin pill by UPLC with accelerated solvent extraction
Author(s) -
Gao Xin,
Yang XiuWei,
Marriott Philip J.
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000169
Subject(s) - coptisine , palmatine , jatrorrhizine , chromatography , rutaecarpine , coptis chinensis , evodiamine , chemistry , berberine , coptis , high performance liquid chromatography , formic acid , herb , extraction (chemistry) , column chromatography , traditional medicine , medicinal herbs , medicine , organic chemistry , alternative medicine , traditional chinese medicine , pathology
A rapid, sensitive, and accurate ultra‐performance liquid chromatography‐UV detection method was developed and validated for simultaneous determination of seven active alkaloids, berberine, palmatine, coptisine, jatrorrhizine, epiberberine, evodiamine, and rutaecarpine, in the Coptis‐Evodia herb couple and the Zuojin pill preparation. The original validated method had a total time of about 11 min, which was reduced to 4.7 min by further optimization of gradient elution conditions with eluent A comprising water/methanol/formic acid (v/v/v, 90:10:0.1) and eluent B ACN. An accelerated solvent extraction method was used for the rapid extraction of herb products and pill preparations. Linear behavior over the investigated concentration ranges was observed, with values of r 2 greater than 0.9987 for all analytes. Experiment results were satisfactory for both intra‐day and inter‐day precision, and method accuracy. Method recovery ranged from 95.7 to 103.5% based on pure reference standards. The validated method was successfully applied to determine amounts of bioactive alkaloid compounds in five samples, in the Coptis‐Evodia herb couple, and the commercial Zuojin pill products. The developed method achieved baseline resolution for all seven main basic compounds and is suitable for use as a routine procedure for the rapid identification and quantification of basic compounds in the Coptis‐Evodia herb couple and related products.