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Tris(hydroxymethyl)aminomethane‐functionalized silica particles and their application for hydrophilic interaction chromatography
Author(s) -
Bui Nhat Thi Hong,
Verhage Jeroen J.,
Irgum Knut
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000154
Subject(s) - glycidyl methacrylate , chemistry , tris , hydroxymethyl , polymer chemistry , dimethylformamide , silica gel , methacrylate , column chromatography , polymerization , atom transfer radical polymerization , polymer , organic chemistry , biochemistry , solvent
A new method is presented for synthesizing a highly hydrophilic silica‐based material for use in hydrophilic interaction chromatography. Porous silica particles used as a starting substrate were modified with 3‐bromopropyl trichlorosilane and grafted with glycidyl methacrylate by controlled (“living”) atom transfer radical polymerization in order to introduce an oxirane‐carrying reactive tentacle layer on the silica surface. The grafted material was thereafter subject to an oxirane ring opening reaction with tris(hydroxy‐methyl)aminomethane in dimethylformamide to yield a polymer‐bound equivalent of the well known and highly hydrophilic “TRIS” buffering substance. Chemical characterization was done by diffuse reflectance FT‐IR, X‐ray photoelectron spectroscopy, elemental analysis, and 1 H NMR. Porosity and surface area examination was done with Brunauer–Emmett–Teller. Chromatographic application of the material was evaluated by separations of nucleic bases, small organic acids, and common nucleotides under mixed hydrophilic interaction chromatography and weak anion exchange conditions.