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MEKC determination of IgG in human serum via a pH‐mediated acid stacking method
Author(s) -
Wu YiWei,
Liu JunFeng,
Deng ZhenLi,
Zhang Jing,
Jiang Feng,
Xiong Kun,
Zhang Haili
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000144
Subject(s) - chemistry , chromatography , stacking , detection limit , electrokinetic phenomena , adsorption , solid phase extraction , matrix (chemical analysis) , analyte , extraction (chemistry) , organic chemistry
An on‐column preconcentration technique, pH‐mediated acid stacking, was used in this study to improve the sensitivity of MEKC‐UV analysis of IgG in human serum. Various parameters affecting pH‐mediated acid stacking were optimized systematically. To eliminate the matrix interferences of human serum and to combine the sample pretreatment procedure with the detection methodology, silica‐coated Fe 3 O 4 magnetic nanoparticles modified with N ‐(2‐aminoethyl)‐3‐aminopropyl‐trimethoxysilane were prepared and employed as solid phase extraction adsorbent to remove the abundant HSA from human serum. HSA was quantitatively removed by silica‐coated Fe 3 O 4 magnetic nanoparticles modified with N ‐(2‐aminoethyl)‐3‐aminopropyl‐trimethoxysilanes without retaining IgG at pH 9.3. Under the optimum conditions, the sensitivity of IgG was improved 40.3‐fold using a 100 s electrokinetic injection as compared with a 6 s hydrodynamic injection. The detection limit of IgG was found to be 0.1 mg/L, and the proposed method was successfully applied for the determination of IgG in human serum with satisfactory results.