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Rapid monitoring of arrays of amino acids in clinical samples using capillary electrophoresis with contactless conductivity detection
Author(s) -
Tůma Petr,
Málková Klára,
Samcová Eva,
Štulík Karel
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000137
Subject(s) - analyte , chromatography , chemistry , capillary electrophoresis , amino acid , capillary action , conductivity , electrolyte , amino acid analysis , acetic acid , detection limit , analytical chemistry (journal) , biochemistry , materials science , electrode , composite material
CE with contactless conductivity detection has been used to separate 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the tested amino acids can be mutually separated in 0.5–10 mol/L acetic acid electrolytes. The time of analysis does not exceed 6 min and the LODs vary from 0.1 to 1.7 μmol/L. The CVs lie within the intervals 0.01–0.4% and 0.9–4% for the migration times and the analyte peak areas, respectively. The procedure has been successfully applied to the determinations of the whole amino acid spectra in blood plasma, urine, saliva and cerebrospinal fluid samples.