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Modeling the electrophoresis and transport of peptides: The effective sphere model and complex formation
Author(s) -
Allison Stuart A.,
Pei Hongxia,
Allen Michelle,
Brown Jocelyn,
Kim ChangIl,
Zhen Yang
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000130
Subject(s) - peptide , electrophoresis , chemistry , ionic strength , ionic bonding , surface charge , chemical physics , chromatography , biological system , ion , organic chemistry , biochemistry , aqueous solution , biology
Modeling the electrophoretic mobility of peptides is examined in this study using a “coarse grained” bead model, or B model for short 8 and also a simpler “effective sphere” (ES) model. A comparison between the B and ES models is carried out for peptide models covering a broad range of ionic strength, peptide charge, and peptide length. At ionic strengths lower than approximately 0.013 M, the B and ES models agree to within a few percent. The ES model is much simpler than the B model and is of particular value in certain applications such as complex formation between peptide and other species in the BGE. The mobility behavior of oligoglycine in a borate buffer at high pH can be accounted for when complex formation is included in modeling.