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Ultra‐performance LC MS/MS method for quantification of clopidogrel active metabolite
Author(s) -
Delavenne Xavier,
Basset Thierry,
Zufferey Paul,
Malouk Nora,
Laporte Silvy,
Mismetti Patrick
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000115
Subject(s) - protein precipitation , chromatography , active metabolite , metabolite , chemistry , pharmacokinetics , high performance liquid chromatography , pharmacology , medicine , biochemistry
A sensible ultra‐performance LC–MS method was developed and validated for the quantification of clopidogrel active metabolite in human plasma, with clopidogrel D4 as internal standard. Plasma pretreatment involved a one‐step protein precipitation with acetonitrile. The separation was performed by reverse‐phase chromatography on a C8 column. The method was linear over the concentration range of 1–150 ng/mL. The intra‐ and inter‐day precision values were below 17% and accuracy was from 1.7 to 7.5% at all quality control levels. The lower LOQ was 0.8 ng/mL. Sample analysis time was reduced to 5 min including sample preparation (limited to protein precipitation). The present method was successfully applied to a clopidogrel active metabolite pharmacokinetic study following oral administration to healthy volunteers.