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A rapid method with ultra‐high‐performance liquid chromatography–tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines
Author(s) -
Han Zheng,
Liu Xuesong,
Ren Yiping,
Luan Lianjun,
Wu Yongjiang
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000094
Subject(s) - chromatography , chemistry , high performance liquid chromatography , analyte , mass spectrometry , resolution (logic) , tandem mass spectrometry , acetonitrile , detection limit , artificial intelligence , computer science
A speedy and selective ultra‐HPLC‐MS/MS method for simultaneous determination of deoxynivalenol (DON), 3‐acetyldeoxynivalenol (3‐ADON), 15‐ADON, nivalenol and fusarenon X in traditional Chinese medicines (TCMs) was developed. The method was based on one‐step sample cleanup using reliable homemade cleanup cartridges. A linear gradient mobile‐phase system, consisting of water containing 0.2% aqueous ammonia and acetonitrile/methanol (90:10, v/v) at a flow rate of 0.4 mL/min, and an Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) were employed to obtain the best resolution of the target analytes. [ 13 C 15 ]–DON was used as the internal standard to accomplish as accurate as possible quantitation. The established method was further validated by determining the linearity ( R 2 ≥0.9990), sensitivity (LOQ, 0.29–0.99 μg/kg), recovery (88.5–119.5%) and precision (RSD≤15.8%). It was shown to be a suitable method for simultaneous determination of DON, 3‐ADON, 15‐ADON, nivalenol and fusarenon X in various TCM matrices. The utility and practical impact of the method was demonstrated using different TCM samples.