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Simultaneous determination of thyroxine and triiodothyronine in pharmaceutical formulations using capillary electrophoresis with amperometric detection
Author(s) -
Sun Yan,
Zhao Xiaoyin,
Li Ping,
Shi Guoyue,
Zhou Tianshu
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000077
Subject(s) - amperometry , chemistry , capillary electrophoresis , analyte , chromatography , detection limit , triiodothyronine , analytical chemistry (journal) , electrode , electrochemistry , biochemistry , hormone
A simple, reliable and repeatable method, based on CE with amperometric detection has been developed for the simultaneous separation and determination of thyroxine (T 4 ) and 3,3′,5‐triiodothyronine (T 3 ). A carbon disk electrode was used as working electrode in this system and exhibited a good response at 1.05 V ( versus Hg/Hg 2 Cl 2 , 3 mol/L KCl) for two analytes. The CE separations were investigated in detail. At 21 kV of applied voltage, the two compounds were separated within 11 min in 0.05 mol/L Na 2 B 4 O 7 ‐NaOH buffer (pH 11.3). And the detection limits were as low as 1.0×10 −7 mol/L for T 4 and 8.5×10 −8 mol/L for T 3 ( S/N =3), respectively. The proposed method was applied to analyze T 4 and T 3 in pharmaceutical formulations, and the results were satisfactory.