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The use of liquid phase deposition prepared phosphonate grafted silica nanoparticle‐deposited capillaries in the enrichment of phosphopeptides
Author(s) -
Wu JianHong,
Zhao Yong,
Li Ting,
Xu Cong,
Xiao Kuang,
Feng YuQi,
Guo Lin
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201000029
Subject(s) - phosphopeptide , chromatography , chemistry , phosphonate , capillary action , extraction (chemistry) , solid phase extraction , nanoparticle , solid phase microextraction , deposition (geology) , capillary electrophoresis , analytical chemistry (journal) , mass spectrometry , materials science , nanotechnology , gas chromatography–mass spectrometry , peptide , organic chemistry , biochemistry , paleontology , sediment , composite material , biology
In our current work, we describe how open tubular‐immobilized metal‐ion affinity chromatography (OT‐IMAC) capillary columns connected to a solid phase microextraction (in‐tube SPME) device can be used for the enrichment of phosphopeptides. A phosphonate modified silica nanoparticle (NP)‐deposited capillary was prepared by liquid phase deposition (LPD), and used for the immobilization of Fe 3+ , Zr 4+ or Ti 4+ . The enrichment capacities of three different OT‐IMAC capillary columns were compared by using tryptically digested α‐casein as sample. The improved extraction efficiency in our technique was demonstrated by comparing to a directly modified capillary, and a comparison of phosphopeptide extraction from simple and complex samples was tested for both modes. Our results show that the NP‐IMAC‐Zr 4+ capillary column can be used to selectively isolate phosphopeptides from real samples, and can enrich for β‐casein phosphopeptides from concentrations as low as 1.7×10 −9 M.