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Monitoring of mutarotation of monosaccharides by hydrophilic interaction chromatography
Author(s) -
Pazourek Jiří
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900880
Subject(s) - mutarotation , chemistry , anomer , monosaccharide , calibration curve , chromatography , furanose , analytical chemistry (journal) , calibration , pyranose , stereochemistry , detection limit , organic chemistry , physics , ring (chemistry) , quantum mechanics
Calibration based on the “single‐point calibration method”, a simple exponential transformation of the response function of an evaporative light scattering detector was improved and applied to analysis of selected saccharides under hydrophilic interaction chromatography mode (a polar phase LiChrospher100 DIOL, mobile phase acetonitrile/water). The improved approach to the calibration procedure yielded a calibration curve with an excellent linearity (quality coefficient <5%). This quantitative evaluation of chromatograms of D ‐galactose suggested that not only anomers but even pyranose and furanose forms of the anomers could be resolved – the resulting calculations of abundance of the anomeric form strongly correlated with data from the literature obtained mostly by NMR studies (analogous results were also obtained for D ‐arabinose, D ‐glucose, and D ‐mannose). Because of the rapid separation (retention time less than 10 min), the observed correlation enabled to monitor anomeric conversion (mutarotation) of monosaccharides.