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Simultaneous determination of yohimbine, sildenafil, vardenafil and tadalafil in dietary supplements using high‐performance liquid chromatography‐tandem mass spectrometry
Author(s) -
Zhang Ying,
Huang Zhiqiang,
Ding Li,
Yan Hongfei,
Wang Meiling,
Zhu Shaohua
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900841
Subject(s) - vardenafil , tadalafil , sildenafil , chromatography , chemistry , tandem mass spectrometry , yohimbine , mass spectrometry , liquid chromatography–mass spectrometry , medicine , biochemistry , receptor , antagonist
A simple and sensitive method was developed for determination of illegal adulterants (yohimbine, sildenafil, vardenafil and tadalafil) in dietary supplements by HPLC‐MS/MS. The separation was achieved on a C 18 column with the mobile phase consisting of acetonitrile and 0.1% acetic acid aqueous solution with a gradient elution at a flow rate of 0.5 mL/min. The analytes were quantified and identified by two characteristic transitions using the multiple‐reaction monitoring mode. The recoveries of the analytes ranged from 77.5 to 109.3% with the RSD less than 8.1% ( n =6). The method has been successfully applied to screen illegal adulterations of natural dietary supplements.