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The impact of silica gel pore and particle sizes on HPLC column efficiency and resolution for an immobilized, cyclodextrin‐based, chiral stationary phase
Author(s) -
Qin Qing,
Zhang Sheng,
Zhang WeiGuang,
Zhang ZhenBin,
Xiong YaJin,
Guo ZiYuan,
Fan Jun,
RunZheng Sheng,
Finlow David,
Yin Yong
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900831
Subject(s) - chemistry , silica gel , particle size , chromatography , resolution (logic) , phase (matter) , cyclodextrin , high performance liquid chromatography , particle (ecology) , stationary phase , chiral column chromatography , chiral stationary phase , analytical chemistry (journal) , organic chemistry , oceanography , artificial intelligence , computer science , geology
Abstract In this study, the CD derivative, mono (6 A ‐azido‐6 A ‐deoxy)‐ per ( p ‐chlorophenylcarbamonylated) β‐CD was chemically immobilized onto the surface of an amino‐functionalized silica gel with different pore (100, 300, and 500Å) and particle (3, 5, and 10 μm) sizes to obtain novel chiral stationary phases. The impact of pore and particle size on the amount of immobilized Ph‐β‐CD, column performance, and enantioselectivity was investigated by evaluating the separation of a variety of racemates in both the normal‐ and the reversed‐phase modes. Experimental results revealed that the retention factor and resolution of racemates generally decreased with increasing pore size; the column prepared with the smallest (3 μm) silica gel particle size gave the best column performance and enantioselectivity in both the normal‐ and the reversed‐phase modes.