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Hydrostatic countercurrent chromatography and ultra high pressure LC: Two fast complementary separation methods for the preparative isolation and the analysis of the fragrant massoia lactones
Author(s) -
Urbain Aurélie,
Corbeiller Pierre,
Aligiannis Nektarios,
Halabalaki Maria,
Skaltsounis AlexiosLeandros
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900818
Subject(s) - chemistry , chromatography , countercurrent chromatography , hexane , methanol , fractionation , solvent , hydrostatic pressure , high performance liquid chromatography , organic chemistry , physics , thermodynamics
Using a one‐step preparative hydrostatic countercurrent chromatography method, the fragrant massoia lactones were purified from the crude massoia bark oil, in less than 3 h. The fractionation was performed with the biphasic solvent system c ‐hexane–methanol–water (10:9:1, v/v/v), leading to target compounds with purity over 96%, as determined by GC‐MS and ultra high pressure LC‐MS analyses. Together with C‐10, C‐12 and C‐14 massoia lactones, two other aromatic compounds used in perfumes, benzyl benzoate and benzyl salicylate, were also obtained as pure compounds. In parallel, an easy and efficient ultra high pressure LC method was developed for the ultra‐fast analysis of massoia lactones, as an alternative to long GC‐MS methods.