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Simultaneous determination of substrate and product in the process of preparation of valienamine by capillary zone electrophoresis
Author(s) -
Wei XiaoDong,
Zhao WenJie,
Gu Min,
Zhao Bo,
Yao RongYing
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900813
Subject(s) - acarbose , capillary electrophoresis , chromatography , chemistry , phosphoric acid , calibration curve , substrate (aquarium) , detection limit , linear range , quantitative analysis (chemistry) , enzyme , biochemistry , organic chemistry , biology , ecology
A simple and rapid CZE method was established for the simultaneous determination of valienamine, acarbose and validamycin A, using a 20‐kV CZE with the detection wavelength of 193 nm and 50 mM phosphoric acid–20 mM Tris (pH 5.3) as a running buffer. The calibration curves of valienamine, acarbose, and validamycin A showed a good linear relationship at a concentration range of 5–1000 μg/mL. The detection limits of valienamine, acarbose, and validamycin A were 0.3, 0.6, and 0.6 μg/mL, respectively, and the average recoveries of each of the above were 99.9, 99.5, and 100.3%. The method has been successfully applied for simultaneous determination of substrate and product in the process of preparation of valienamine.