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Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography
Author(s) -
Dadáková Eva,
Kalinová Jana
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900809
Subject(s) - micellar electrokinetic chromatography , quercetin , chromatography , chemistry , glycoside , capillary electrophoresis , electrokinetic phenomena , rutin , organic chemistry , antioxidant
A simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze‐dried homogenous material in 50% v/v methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using SPE. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, LOD and LOQ, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99 400 mg/kg in F. esculentum , 108 000 mg/kg in F. tataricum ). The free quercetin occurs in flowers and achenes of F. esculentum , whereas flowers and achenes of F. tataricum contained quercitrin.